Transcript identification by analysis of short sequence tags--influence of tag length, restriction site and transcript database

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Transcript identification by analysis of short sequence tags--influence of tag length, restriction site and transcript database.

There exist a number of gene expression profiling techniques that utilize restriction enzymes for generation of short expressed sequence tags. We have studied how the choice of restriction enzyme influences various characteristics of tags generated in an experiment. We have also investigated various aspects of in silico transcript identification that these profiling methods rely on. First, anal...

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Transcript Sequence Registry

In many computational genomic studies, including large-scale expression profiling, it is useful to have a comprehensive collection of transcript sequences. The Reference Sequence [NCBI, 1999] project at NCBI is an effort to identify a non-redundant set of full-length mRNA sequences contained within GenBank [Benson et al., 1999] entries. Currently, RefSeq consists of approximately 6,300 human mR...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 2003

ISSN: 1362-4962

DOI: 10.1093/nar/gkg313